Polydiacetylene based sensors have been used for a variety of sensing applications. Here we demonstrate that diacetylene-containing amphiphiles could be spray processed to provide unique sensor coatings that may be applied to a variety of material surfaces. Utilizing an airbrushing technique, we examined processing parameters to yield spray-on sensors for detecting UV, temperature, as well as chemical/solvent targets depending on the pendant side chain chemistry of the resulting polydiacetylene coating. We observed that the concentration, carrier solvent, and UV exposure time had a significant effect on the colorimetric response of the sprayed on coating with better responses observed at higher amphiphile concentration and shorter polymerization times. We expect this approach to generating spray-on responsive coatings will provide a widely deployable means for detection that may be customized for a variety of sensing applications.