Electrospun cellulose acetate nanofibers were synthesized and deacetylated by alkaline hydrolysis. Au@AgNPs were synthesized and decorated on the surface of cellulose nanofibers. The Au@AgNPs was decorated by dipping method on the surface of cellulose nanofibers to construct effective wound dressing material. Au@NPs and Au@AgNPs/ cellulose nanofibers were characterized by scanning electron microscope and transmission electron microscope. Moreover, antibacterial activity was assessed against E. coli and S. aureus finding Au@AgNPs/cellulose excellent antimicrobial material. Moreover the wound healing experiment was carried out by using Au@AgNPs/cellulose as a wound dressing material, and determining great results.