The structural composition and functionality of bone tissues has been significantly impacted due to progressive ageing, thereby necessitating effective strategies that are not just about temporary cure but about permanent regeneration of tissues, one such being regenerative medicine. Of the several novelties in the field, the provocation of osteogenesis involving nanoparticles has gained significant attention for their heightened efficiency and cost effectiveness. Magnetic materials in particular have been increasingly paid attention to owing to their edge over stability, non-toxicity and feasibility to spatially track using MRI. The present study involves the synthesis of Zn and Ni doped ferrite ((Zn, Ni)-ferrite) nanoparticles involving chemical co-precipitation method. The materials were evaluated against MC3T3 cells towards ROS induced osteogenic differentiation properties, and the results implied very less toxicity even at concentration surpassing 50 µg/ml in addition to the void of necrosis after treatment with 2.5 and 5 µg/ml of (Zn, Ni)-ferrite. FACS results implied 1% cell staining with propidium iodide after 24 h exposure at 2.5 µg/ml concentrations of (Zn, Ni)-ferrite, thereby substantiating (Zn, Ni)-ferrite as a potential biomaterial in favor of regenerative medicine.