A number of glycosidase enzymes have been isolated and identified in healthy fresh leaves of turnip. Myrosinase (beta-thioglucosidase, EC. 3.2.3.1), disaccharase (beta-fructofuranosidase, EC. 3.2.1.26), and beta-amylase (alpha-1,4-glucan maltohydrolase, EC 3.2.1.2) have been isolated and purified in powder form. The purification methods were salting out with ammonium sulfate, DEAE-cellulose column chromatography, hydroxylapatite batch chromatography, and gel filtration through Sephadex G-200. Four isoenzymes of myrosinase enzyme could be isolated. The most active one was purified (131.3 times) and found to have an SA of 19.7 U/mg. An enzyme causing hydrolysis of amylose and glycogen has been isolated and partially purified. It had an SA of 22.6 U/mg and 49.1-fold purification. Seven isoenzymes of disaccharase were isolated, but only one was purified (C) with SA of 1448.5 U/mg and 1316.8-fold purification. The yield of the purified myrosinase and disaccharase enzymes was 3.68 and 0.5 mg, respectively, from 100 gm dry wt of turnip leaves. Confirmation of purity with disk electrophoresis was performed. A single sharp band was obtained for each pure enzyme by disk electrophoresis. The chromatographic analysis of the hydrolytic end product of the beta-amylase indicated the presence of maltose. This results confirm the beta-form of amylase enzyme.e-g