A serum starvation culture of hybridoma cells is desirable because serum starvation stops cell cycling at the G(1) phase, where hybridoma cells produce more antibody in the ether phases, However, serum starvation induces apoptosis, a type of cell death, forcing the culture to die out. In this research, the authors aimed at inhibition of cell death during serum starvation culture. A hybridoma cell line bcl-2 was transfected with bcl-2, an apoptosis suppressing gene, and named 2 E 3-bcl 2. Then the cells were screened in serum starvation culture to obtain 2 E 3-bcl 2-LS. The expression level of Bcl-2 protein in 2 E 3-bcl 2-LS was higher by 40% than that in 2 E 3-bcl 2. In serum starvation culture, 2 E 3-bcl 2-LS survived longer by two days and produced twice as much antibody as the wild type.