Bacillus polymyxa CECT 155 produces an extracellular neopullulanase activity that degrades pullulan to panose. This activity was stimulated by the presence of pullulan in the culture, and repressed by glucose. The apparent mot wt determined for the enzyme was 58 kDa. The optimum pH and temperature for neopullulanase activity were pH 6.0 and 50 degrees C, respectively. The enzyme was stable in a pH range of 4.0-8.0, and temperatures up to 60 degrees C. These properties make it suitable for the saccharification processes in the starch industries.