Applied Biochemistry and Biotechnology, Vol.77-79, 67-81, 1999
Modeling the enzymatic hydrolysis of dilute-acid pretreated douglas fir
Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7-36 filter paper units [FPU]/g cellulose) and solids concentration (7-18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect even at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.