The main goal of the present work is to gain insight into the mechanisms and forces that govern the adsorption of F(ab’)(2) fragments onto different polymer surfaces. We have employed F(ab’)(2) fragments obtained by pepsin digestion of rabbit polyclonal IgG. Colloidal polystyrene particles with different superficial characteristics have been used as polymer carriers. These latex particles differ in their hydrophobic/hydrophilic character, surface charge density, and the nature of the ionic groups located at their solid-water interface. We have checked that the most important driving force during the F(ab’)(2) adsorption is the hydrophobic one. However, when adsorption isotherms are performed in media of low ionic strength, the electrostatic interaction plays an important role. First, adsorption experiments carried out, followed by both desorption studies and electrokinetic characterization of the F(ab’)(2)-latex complexes. The results of the electrokinetic characterization are in good agreement with those obtained from the stability studies. The research presented in this paper throws light on interesting aspects important when developing immunoassay reagents.