Densely packed monolayers of latex particles with a diameter of 70 nm were obtained by means of the Langmuir-Blodgett method with a polymer colloid directly used as a subphase. Different kinds of spreading molecules were tested to transfer the latex particle monolayer. Cospreading molecule monolayers were proved to be the best choice. The pH value of the subphase was a key factor in controlling the interaction between the particles and the spreading molecule monolayers. The pi-A isotherms were in good agreement with transmittance electron microscopy images, indicating that in the case of acidic condition, the interattraction force was strong enough to make polymer particles form a monolayer and to pull it onto the substrate.