We observed, by using a quartz crystal microbalance, linear oligonucleotides binding selectively to the planar nucleobase lipid monolayer at the air-water interface and hybridization between two linear oligonucleotides in aqueous solution. Kinetics of DNA hybridization at the interface could be controlled by changing the distance and orientation between nucleobases in the monolayer and compared with those for conventional hybridization in the aqueous solution.