The objective of this work is to test the very recent Levin＇s theory for charged colloids (Levin, Y.; Barbosa, M. C.; Tamashiro, M. N. Europhys. Lett. 1998, 41 (2), 123). To accomplish this, the fraction of condensed ions has been experimentally obtained from electrophoretic mobility data and surface charge values. Latexes with different functionalities, surface charges, and sizes were selected in order to support the universality of the ion condensation effect. The good agreement between charge and fraction of condensed ions confirms Levin＇s theory and makes ion condensation a clear candidate for explaining the observed insensitivity of the electrophoretic mobility to surface charge variations, for sufficiently large charge.