The charge transfer (CT) properties were examined in the mixed and the alternate-layer Langmuir-Blodgett (LB) films of 2-methyl-5-octadecyl-1,4-dihydroxybenzene (C(18)HQ) and 2-methyl-5-octadecyl-1,4-benzoquinone (C(18)Q). Intermolecular CT took place in both types of LB films. The CT band between C(18)HQ and C(18)Q appeared at 590 nm suggested the neutral CT state as in the case of unsubstituted quinhydrone. The direction of CT was examined by the polarized spectra. The CT state was accompanied by hydrogen bonding, which was confirmed by the hydrogen bonded and non-hydrogen bonded C=O stretching bands appeared in the IR spectra.