The construction of a novel baker's yeast variety via traditional genetic techniques is described. The phenotype was designated ''Lti'' (''Low temperature inactive''). Lti mutations with the desired characteristics within a genetically well-defined haploid laboratory strain of Saccharomyces cerevisiae were isolated, and two different approaches were taken to obtain baker's yeast strains, which exhibit reduced fermenting activity at refrigeration temperatures. In a first approach, a chosen Lti strain carrying mutation lti9 was combined with other laboratory strains carrying defined MAL alleles. In a second approach, the same lti mutation was introduced in the genetic background of polyploid commercial baker's yeast strains that harbor important ''industrial'' properties. Lti strains arising from both approaches were characterized with specifically developed screening procedures. Strains of the ''academic'' Lti strain family displayed between 85% and 92% of the biomass yield of a commercial reference strain, whereas strains of the ''industrial'' Lti strain family showed a variation between 60% and 115%. Lti strains from both families varied strongly among each other in their activity in model doughs: at KC they displayed activities between 5% and 30%, and at 30degreesC between 40% and 113% of a commercial reference baker's yeast strain.