Aqueous extracts of fungal biomass of Aspergillus niger, A. flavus, Penicillium notatum, Fusarium oxysporum and the filtrates of their culture media were analysed for elicitation capability to enhance anthocyanin production in callus cultures of Daucus carota. The mycelial extract of A. flavus at the 2.5% level gave maximum elicitation, which resulted in a two-fold increase in anthocyanin with maximal productivity of 23.7% on a dry weight basis. Whereas the media filtrates of A, flavus induced a 1.25-fold increase in anthocyanin production with a yield of 20.6% on a dry weight basis, P. notatum and F. oxysporum were not as effective as A. flavus or A. niger. The contact time and the concentration required for maximal elicitation of anthocyanin differed with the type of elicitor used. Qualitative analysis of anthocyanins revealed the presence of cyanidin glycosides in control and elicited cultures.