Applied Microbiology and Biotechnology, Vol.43, No.1, 65-69, 1995
Molecular-Cloning and Expression in Escherichia-Coli of Bleomycin-Resistance Gene from a Methicillin-Resistant Staphylococcus-Aureus and Its Association with Is431 Mec
A gene that confers bleomycin resistance was cloned from the chromosomal DNA of methicillin-resistant Staphylococcus aureus (MRSA) B-26 into the plasmid pUC18. It is of chromosomal origin rather than plasmid and exists in the chromosome making a cluster with the kanamycin-resistance gene. We found that the nucleotide sequence of the bleomycin-resistance gene from the chromosome of MRSA B-26 is identical to that from a staphylococcal plasmid, pUB110. The partial sequence of IS431mec was also found upstream from the DNA fragment containing the bleomycin- and kanamycin-resistance genes.