Using 4,4＇-dimethoxybenzhydrol as soluble mimic of the reduced poly(aryl ether ether ketone) (PEEK) monomer unit, we established the best experimental conditions to replace the hydroxyl group with an amine function, on the one hand, and to fix an amino acid residue, on the other hand. The selected strategies were then adapted to the surface functionalization of the PEEK-OH film obtained by reduction of the PEEK precursor. Thus, reaction with phenylcarbamate followed by LiOH hydrolysis gave the PEEK-NH2 film characterized by theta(w), multiple internal reflection (MIR) and X-ray photoelectron specstroscopy, (XPS). The PEEK-NH2 was further derivatized with 1,3-propanesultone. Reaction of PEEK-OH with N＇-(9-fluorenylmethoxvcarbonyl) (L) glutamine followed by piperidine deprotection gave the PEEK-glutamine sim characterized by theta(w), MIR and XPS.