Yeasts were isolated from exudates of algarrobo trees from nine sites in northwest Argentina, three in the Chaco region, from pods of algarrobo and acacia, collected in the Quebrada de Cafayate, and from rot pockets in decaying columnar cacti at Cachi (Parc Nacional des Cardons), and at Las Ruinas de Quilmes. Most of the yeasts isolated from exudates of algarrobo were identified as Bullera variabilis, Candida famata, Cryptococcus albidus and other Cryptococcus species, Debaryomyces hansenii, Pichia angusta (Hansenula polymorpha), Pichia ciferrii, Pichia farinosa and Torulaspora delbrueckii. Other Candida, Kluyveromyces and Pichia species were found. Most species were osmotolerant. The high sugar content of the exudates influenced the nature of the yeast species present. The pods contained Cryptococcus spp., Candida famata and Candida ciferrii spp. Half the species isolated from rotting cactus were Cryptococcus species. Pichia membranaefaciens and Torulaspora delbrueckii were also isolated. Of 553 cultures investigated, 49 utilized methanol.