This paper describes a new nondestructive technique for detecting internal stresses in coatings using time resolved fluorescence. The measurement principle is based upon an experimental result that : the decay time of fluorescence from poly(3-octylthiophene), P3OT, dispersed in uniaxially-stretched polymer films decreases with increasing tensile stress acting on the films. Internal stresses in the clear coat and in the base coat of a multilayer structure, which was composed of electrodeposited coat, surface coat, base coat, and clear coat, were estimated from the decay time of fluorescence from P3OT in both coats. The order of internal stresses in the clear coat and base coat of the multilayer system was I MPa. When the coatings were piled up, the internal stress decreased as the distance from a metal substrate was increased. It was found that moisture and temperature influenced internal stresses in the clear coat father than in the base coat. Internal stress in tile clear coat, which was one layer coated on a metal substrate, was measured by the traditional bimetallic mettled or by the time resolved fluorescence technique. Comparing both methods, it was concluded that the time resolved fluorescence technique gave reliable values for internal stresses in coatings.