Tempe fermentations using mixed cultures of Rhizopus oligosporus MS5, R. oryzne EN, Citrobacter freundii, and Brevibacterium epidermidis were investigated. Consumption of 150 g tempe, produced with a pure fungal mixed culture out of strains MS5 and EN, is sufficient to cover the daily requirements of niacin, vitamin K, ergosterol, and tocopherol as well as half of the daily requirement of pyridoxine, riboflavin, and biotin. Moreover, one-fourth of the recommended amount of folate is supplied. Supplementation of the fungal inoculum with C. freundii results in tempe enriched with vitamin B-12. Menachinone was produced as a typical bacterial vitamin K derivative. Metabolic activity of C. frendii led to an additional decrease of the alpha-galactosides stachyose and raffinose compared to pure fungal fermentations. No bacterial formation of factor 2 could be observed.