The validity of the universal calibration concept in tetrahydroforan (THF) and in dimethylacetamide (DMAC) is explored in the low molecular weight region for molecular weights less than 10,000 daltons. A variety of calibrant polymer types and column packings are studied utilizing a single capillary GPC-viscometer (DV)/differential refractometer (DRI) chromatographic system. Use is made of the Sanayei-O'Driscoll-Rudin single parameter universal calibration model based on K-theta to assess the validity of the universal calibration concept.