Applied Microbiology and Biotechnology, Vol.47, No.6, 734-741, 1997
Nitrification, Denitrification, and Dechlorination in Bleached Kraft Pulp-Mill Waste-Water
This study deals with combining the biological removal of organic halogens with the removal of nitrogen from bleached kraft pulp mill wastewater in fluidized-bed reactors under nitrifying and denitrifying conditions, Untreated and biotreated bleached kraft pulp mill wastewaters had no detrimental effect on nitrification or denitrification. The nitrifying biofilm reactor, pregrown on synthetic inorganic feed with ammonia, removed without a lag phase adsorbable organic halogens [7.2 mg Cl (g biomass volatile solids)(-1)day(-1)] from bleached kraft pulp mill wastewater and selected chlorophenols from synthetic wastewater. Electron microscopical examination of the biofilm showed that bacteria, morphologically similar to the nitrifying species Nitrosomonas or Nitrobacter, and Nitrosospira were dominant. The denitrifying fluidized-bed reactor, pregrown on nitrate and methanol, denitrified without a lag phase bleached kraft pulp mill wastewater. Under denitrifying conditions, 35% of the total organic carbon content of untreated bleached kraft pulp mill wastewater was removed. The reducing power delivered by untreated bleached kraft pulp mill wastewater for denitrification was 2 mmol electrons/mmol carbon mineralized. Dechlorination under denitrifying conditions was negligible.
Keywords:NITROSOMONAS-EUROPAEA;AMMONIA MONOOXYGENASE;BIOLOGICAL TREATMENT;OXIDIZING BACTERIUM;NITRIFYING BACTERIA;PAPER-INDUSTRY;NITROSOSPIRA;DEGRADATION;OXIDATION