Purification of the Main Manganese Peroxidase Isoenzyme Mnp2 from the White-Rot Fungus Nematoloma-Frowardii B19

Schneegass I; Hofrichter M; Scheibner K; Fritsche W

Applied Microbiology and Biotechnology, Vol.48, No.5, 602-605, 1997

DOI10.1007/s002530051102 Export Citation

Purification of the Main Manganese Peroxidase Isoenzyme Mnp2 from the White-Rot Fungus Nematoloma-Frowardii B19

Schneegass I, Hofrichter M, Scheibner K, Fritsche W

The main manganese peroxidase isoenzyme MnP2 of the South American white-rot fungus Nematoloma frowardii b19 was purified to homogeneity using anion-exchange chromatography (Mono Q) and preparative isoelectric focusing. The purified enzyme has a molecular mass of 44 kDa and a pI of 3.2.

Keywords:PHANEROCHAETE-CHRYSOSPORIUM;LIGNIN DEGRADATION;BASIDIOMYCETE;CHELATORS;CULTURES;PROTEINS;ENZYMES