The use of reverse micellar systems for protein extraction has attracted considerable interest because of their capacity to solubilize specific proteins from dilute aqueous solutions such as fermentation and cell culture media. A forward and back extraction can be used for the recovery of proteins. The conditions for the extraction of alpha-amylase protein have been investigated, with special reference to the recovery of enzymic activity. Protein release depended on aqueous phase pH; the alpha-amylase protein increasing at lower pH values of the back extraction aqueous phase. The enzyme retained its biological activity fairly well inside reverse micelles.