Separation of Cephalosporin-C Acylase and Penicillin-V Acylase from Aeromonas Acy-95 by Chemical Treatment

Ambedkar SS; Deshpande BS; Shewale JG

Process Biochemistry, Vol.32, No.4, 305-307, 1997

DOI10.1016/S0032-9592(96)00059-3 Export Citation

Separation of Cephalosporin-C Acylase and Penicillin-V Acylase from Aeromonas Acy-95 by Chemical Treatment

Several surfactants and solvents were evaluated for the extraction of cephalosporin C acylase from Aeromonas ACY 95. Treatment of the cells with acetone (0.5% v/v) allowed 85% recovery of cephalosporin C acylase with only 10% penicillin V acylase.

Keywords:STRAINS