Nutritional requirements of a mutant strain of Streptomyces clavuligerus for Cephamycin C production were derived by testing various indigenous substrates and their concentrations in production medium. Upon initial screening, six components : tapioca starch (TS), corn steep liquor (CSL), sunflower cake (SFC), K2HPO4, MgSO4 . 7H(2)O and MnSO4 were identified as supporting high production of Cephamycin C. Statistical analysis (ANOVA) of media components indicated that addition of oil cake SFC to the medium enhanced production significantly. SFC controlled foam during fermentation and served as a pH regulator.