Trichoderma koningii UC174 produced a number of exoenzymes when grown in liquid medium containing cell walls from Trichosporon cutaneum SLA09 as the sole carbon source. Exoenzymes were isolated from the culture broth and used to prepare protoplasts from T. cutaneum. An enzyme prepared by bentonite treatment from the original Trichoderma enzyme had less proteinase activity and protoplasts were fairly stable with this product during incubation for 6 h. Neither Cellulase Onozuka RS nor snail gut enzymes from Helix pomatia were able to generate protoplasts either alone or in combination. Protoplasts were produced, however, when commercial chitinase was added to either of these two enzymes. (C) 2000