In this paper, a series of different adsorbents for pseudobiospecific ligand affinity chromatography is presented. They were obtained using ligands as histidine, Cu2+- or Zn2+-chelating iminodiacetic acid and Cibacron Blue F3GA dye, attached to poly(butadiene-hydroxyethylmethacrylate) (PB-HEMA) matrix. The histidine and metal-ion-chelating iminodiacetic acid were attached on matrix through epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (EDGE) as spacers and activators, while dye was directly bound onto the matrix.