A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)(n).(TG)(n) is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)(5) microsatellite located in the 5＇ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the beta-D-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression.