Sporopollenin obtained from Lycopodium clavatum has been modified as an ligand-exchange material. In this study, the possibility of using sporopollenin as a ligand exchanger in the chromatographic separation of amino acids is examined. Since sporopollenin has important advantages (it is stable to chemicals and has a constant mesh size), it can be used as a column packing material. By first treating sporopollenin with 1,2-diaminoethane and then with bromoacetic acid, carboxylated diaminoethylsporopollenin (CMDAE-sporopollenin) was obtained. This new resin was loaded with Co(II) transition element and used as a ligand-exchange material in the separation of amino acids. Using the ligand-exchange technique on Co(II)-loaded carboxylated diaminoethylsporopollenin is a suitable chromatographic method for the separation of most common amino acids.