The continuous separation of glucose and fructose aqueous mixtures was experimentally performed by using a two-section simulated moving bed (SMB) process with Dowex 50W-X12 resin of Ca2+ form as an adsorbent and water as an eluant. The operation mode of the two-section SMB was modified from that of a three-section SMB developed by Barker et al. (1). A plug flow model with velocity-dependent mass transfer resistance was used for estimating the concentration profiles of the two-section SMB process, and the model equation was solved by the numerical method of orthogonal collocation. The two-section SMB suggested in this work was successful for obtaining high fructose corn syrup (55-90% w/w fructose).