As a step towards development of an efficient method for separation of cephalosporin C from fermentation broth, reactive extraction in supported liquid membrane has been investigated using simulated feed solution. Experiments were conducted in a diaphragm type cell with Celgard-2400 membrane as the solid support and Aliquat-336 as the carrier dissolved in butyl acetate as the diluent. Cephalosporin C was permeated across the membrane from an alkaline feed phase of carbonate buffer into an acidic strip solution of acetate buffer via a coupled transport phenomenon involving Cl ions. The permeation process was analyzed from the hypothesis of an overall transport dependance on diffusion across the liquid membrane and total re-extracting in the stripping phase.