The paper presents a new output feedback adaptive control scheme for multi-input and multi-output (MIMO) non-linear systems whose non-linear terms depend only on the output variable based on the backstepping approach with vector form. The assumption on the prior knowledge of the high frequency gain matrix in existing results is reduced and the new required condition for the high frequency gain matrix can be easily checked for certain plants so that the proposed method is widely applicable. This control scheme guarantees the global stability of the closed-loop systems and makes the tracking error be arbitrary small. A simulation example is included to demonstrate the proposed algorithm.