Vegetable cuticular waxes have to be separated from the more valuable essential oils in extraction processes carried out with supercritical fluids (namely CO2), allowing a solvent selectivity by modification of temperature and pressure. The solubility of these waxes in supercritical CO2 can be adequately represented by that of solid n-octacosane. The present work shows that isothermal investigations, carried out with a differential flux calorimeter with adequate cells, allow the solubility and solubilization enthalpy of n-octacosane to be determined. The results concern investigations either at constant pressure and variable supercritical CO2 flow-rate, or at zero flow-rate and increasing pressure. The latter conditions allow the solubility-pressure trend to be obtained, in satisfactory agreement with literature data derived from standard dynamic methods.