A denitrifying consortium capable of transforming carbon tetrachloride (CCl4) was cultured from an aquifer soil sample from the U.S. Department of Energy’s Hanford Site in southeastern Washington State. A mathematical description of the kinetics of CCl4 destruction by this microbial consortium is presented, and its predictions are compared to experimental data. The model successfully predicted the concentrations of acetate, nitrate, nitrite, biomass, and CCl4 for all 12 experiments (a total of 60 concentration-vs.-time data sets). In addition, no statistically significant interactions exist between parameter values and individual test conditions. The ability of the model to predict the results of a treatability test for CCl4 degradation in Hanford groundwater, without adjusting any model parameters, is discussed.