The electron transmission through thin organic films was investigated in order to reveal the effect of the chemical nature of the molecules composing the thin organic layer and the role of the three-dimensional structure of the film on the electron transmission efficiency. By preparing Langmuir-Blodgett layers from two types of amphiphile and a film which is a mixture of both, we could vary the chemical properties of the film and the order in the plane perpendicular to the direction of electron propagation. It was found that the electron transmission yield depends on the chemical nature of the him and varies with the him thickness in a non-trivial way. In the case of the mixed film, the electron transmission is reduced dramatically compared to the films composed from one type of molecule. The results are explained by the long wavelength associated with the low-energy electrons.