Circular dichroism (CD) studies have been utilized to investigate complexation of antibiotic (+)-cephardine with Cr(III), Co(II) and Ni(II) ions in methanol: water (80:20 v/v) solution. The optimum conditions of pH, stoichiometry and time for achieving equilibrium for maximum complexation in these systems are determined. The CD spectra of these complex ions consist of 2-3 Cotton bands between een 16,129-25,994 cm(-1) with differential absorptivity 0.0046-0.423 deg. L mol(-1) cm(-1) and bands are assigned to electronic transitions. The formation constants of these complexes in mixed solvent are reported