The structures of one major and three minor constituents of sterol fraction of the Cyclamen coum have been established as stigmasterol [(22E)-(24S)-24-ethylcholesta-Delta(5,22)-dien-beta-ol] (1), Poriferasterol [22E)-(24R)-24-ethylcholesta-Delta(5,22)-dien-3 beta-ol] (2), stigma-or poriferasta-Delta(3,5,22) triene [(22E)-24-ethylcholesta-Delta(3,5,22)-trien] (3) stigma-or poriferosta-Delta(3,5,7,22)-tetraene [(22E)-24-ethylcholesta-Delta(3,5,7,22)-tetraene] (4) by spectroscopic means (NMR, EI-MS and GC-MS).