Biotechnology and Bioengineering, Vol.51, No.1, 69-78, 1996
Immobilization of Candida-Cylindracea Lipase on Colloidal Liquid Aphrons (Clas) and Development of a Continuous CLA-Membrane Reactor
A novel technique is described for the immobilization of Candida cylindracea lipase in the soapy-shell of colloidal liquid aphrons (CLAs). CLAs consist of a micron-sized solvent droplet surrounded by a thin, aqueous, soapy-film and are stabilized by a mixture of nonionic and ionic surfactants. Retention of lipase within the CLAs is primarily determined by electrostatic interactions between the surface charges on the protein and those of the anionic surfactant used (SDS) because leakage of the lipase from dispersed CLAs was reduced at low continuous phase pH (