화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korea Polymer Journal, Vol.9, No.2, 107-115, April, 2001
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Biocompatibility of Poly(MPC-co-EHMA)/Poly(L-lactide-co-glycolide) Blends
Gilson Khang†, Myoung Kyu Choi, John M. Rhee, Sang Jin Lee1, Hai Bang Lee1, Yasuhiko Owasaki2, Nobuo Nakabayashi2, and Kazuhiko Ishihara3
  • Department of Polymer Science and Technology, Chonbuk National University, Chonju 561-756, Korea
  • 1Biomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O.Box107, Yusung, Taejon 305-606, Korea
  • 2Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan
  • 3Department of Materials Science, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
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Poly(L-lactide-co-glycolide) (PLGA) was blended with poly[ω-methacryloyloxyethyl phosphorylcholine-co-ethylhexylmethacrylate (PMEH)] (PLGA/PMEH) to endow with new functionality i.e., to improve the cell-, tissue- and blood-compatibility. The characteristics of surface properties were investigated by measurement of contact angle goniometer, Fourier-transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) and electron spectroscopy for chemical analysis (ESCA). NIH/3T3 fibroblast and bovine aortic endothelial cell were cultured on control and PLGA/PMEH surfaces for the evaluation of cell attachment and proliferation in terms of surface functionality such as the concentration of phosphorylcholine. Also, the behavior of platelet adhesion on PLGA/PMEH was observed in terms of the surface functionality. The contact angles on control and PLGA/PMEH surfaces decreased with increasing PMEH content from 75° to about 43°. It was observed from the FTIR-ATR spectra that phosphorylcholine groups are gradually increased with increasing blended amount of MPC. The experimental P percent values from ESCA analysis were more 3.28 similar to 7.4 times than that of the theoretical P percent for each blend films. These results clearly indicated that the MPC units were concentrated on the surface of PLGA/PMEH blend. The control and PLGA/PMEH films with 0.5 to 10.0 wt% concentration of PMEH were used to evaluate cell adhesion and growth in terms of phosphorylcholine functionality and wettability. Cell adhesion and growth on PLGA/PMEH surfaces were less active than those of control and both cell number decreased with increasing PMEH contents without the effect of surface wettability. It can be explained that the fibronectin adsorption decreased with an increase in the surface density of phosphorylcholine functional group. One can conclude the amount of the protein adsorption and the adhesion number of cells can be controlled and nonspecifically reduced by the introduction with phosphorylcholine group. Morphology of the adhered platelets on the PLGA/PMEH surface showed lower activating than control and the number of adhered platelets on the PLGA/PMEH sample decreased with increasing the phosphorylcholine contents. The amount of fibrinogen adsorbed on the PLGA/PMEH surface demonstrated that the phospholipid polar group played an important role in reducing protein adsorption on the surface. In conclusion, this surface modification technique might be effectively used PLGA film and scaffolds for controlling the adhesion and growth of cell and tissue, furthermore, bleed compatibility of the PLGA was improved by blending of the MPC polymer for the application of tissue engineering fields.
  1. Agrawal CM, Niederauer GG, Micallef DM, Athanasiou KA, The Use of PLA-PGA Polymers in Orthopedics, in Encyclopedic Handbook of Biomaterials and Bioengineering, Part A, New York, Marcel Dekker, 2, 1055 (1995)
  2. Hollinger JO, Schmitz JP, J. Oral Maxillofac. Surg., 45, 594 (1987)
  3. Williams DF, Mort E, J. Bioeng., 1, 231 (1997)
  4. Bostman O, J. Bone Joint Surg., 73-A(1), 148 (1991)
  5. Agrawal CM, Gabriele PE, Niederauer G, Athanasiou KA, Tissue Eng., 1, 241 (1995)
  6. Cima LG, Ingber DE, Vacanti JP, Langer R, Biotechnol. Bioeng., 38, 145 (1991) 
  7. Mikos AG, Sarakinos G, Leite SM, Vacanti JP, Langer R, Biomaterials, 14, 323 (1993) 
  8. Wald HL, Sarakinos G, Lyman MD, Mikos AG, Vacanti JP, Langer R, Biomaterials, 14, 270 (1993) 
  9. Mikos AG, Bao Y, Cima LG, Ingber DE, Vacanti JP, Langer R, J. Biomed. Mater. Res., 27, 183 (1993) 
  10. Giodano GG, Thomson RC, Ishaug SL, Mikos AG, Cumber S, Garcia CA, LahiriMunir D, J. Biomed. Mater. Res., 34, 87 (1997) 
  11. Grande DA, Halberstadt C, Naughton G, Schwartz R, Manji R, J. Biomed. Mater. Res., 34, 211 (1997) 
  12. Wang HT, Palmer H, Linhardt RJ, Flanagan DR, Schmitt E, Biomaterials, 11, 679 (1990) 
  13. Carrio A, Schwach G, Coudane J, Vert M, J. Control. Release, 37, 1139 (1999)
  14. Schmidt C, Wenz R, Nies B, Moll F, J. Control. Release, 37, 83 (1995) 
  15. Cho JC, Khang G, Rhee JM, Kim YS, Lee JS, Lee HB, Korea Polym. J., 7(2), 79 (1999)
  16. Khang G, Cho JC, Lee JW, Rhee JM, Lee HB, Bio-Med. Mater. Eng., 9(1), 49 (1999)
  17. Khang G, Lee HB, Park JB, Bio-Med. Mater. Eng., 5, 245 (1995)
  18. Khang G, Lee HB, Park JB, Bio-Med. Mater. Eng., 5, 278 (1995)
  19. Khang G, Jeong BJ, Lee HB, Park JB, Bio-Med. Mater. Eng., 5, 259 (1995)
  20. Khang G, Jeon JH, Lee JW, Cho SC, Lee HB, Bio-Med. Mater. Eng., 7(6), 357 (1997)
  21. Hoffman AS, Annal. New York Acad. Sci., 516, 96 (1987) 
  22. Sakakida M, Nishida K, Schichiri M, Ishihara K, Nakabayashi N, Sens. Actuators B-Chem., 319 (1993)
  23. Yoneyama T, Ishihara K, Nakabayashi N, Ito M, Mishima Y, J. Biomed. Mater. Res., 43, 15 (1998) 
  24. Ishihara K, Shinozuka T, Hanazaki Y, Iwasaki Y, Nakabayashi N, J. Biomater. Sci.-Polym. Ed., 10, 271 (1999)
  25. Ishihara K, Ueda T, Nakabayashi N, Polym. J., 22, 355 (1990) 
  26. Ishihara K, Oshida H, Endo Y, Watanabe A, Ueda T, Nakabayashi N, J. Biomed. Mater. Res., 27, 1309 (1993) 
  27. Tegoulia VA, Yu XY, Cooper SL, Trans. Soc. Biomater., 24, 74 (1998)
  28. Marra KG, Winger TM, Hanson SR, Chaikof EL, Macromolecules, 30(21), 6483 (1997) 
  29. Kohler AS, Parks PJ, Mooradian DL, Rao GH, Furcht LT, J. Biomed. Mater. Res., 32, 237 (1996) 
  30. van der Heiden AP, Willems GM, J. Biomed. Mater. Res., 40, 195 (1998) 
  31. Baumgartner JN, Yang CZ, Cooper SL, Biomaterials, 18, 831 (1997) 
  32. Baumgartner JN, Cooper SL, J. Biomed. Mater. Res., 40, 660 (1998) 
  33. Ishihara K, Fukumoto K, Aoki J, Nakabayashi N, Biomaterials, 13, 145 (1997) 
  34. Fukumoto K, Ishihara K, Takayama R, Aoki J, Nakabayashi N, Biomaterials, 13, 235 (1997)
  35. Iwasaki Y, Kurita K, Ishihara K, Nakabayashi N, J. Biomater. Sci.-Polym. Ed., 6, 447 (1994)
  36. Iwasaki Y, Kurita K, Ishihara K, Nakabayashi N, J. Biomater. Sci.-Polym. Ed., 8, 151 (1996)
  37. Ishihara K, Tanaka S, Furukawa N, Kurita K, Nakabayashi N, J. Biomed. Mater. Res., 32, 391 (1996) 
  38. Ishihara K, Shibata N, Tanaka S, Iwasaki Y, Kurosaki T, Nakabayashi N, J. Biomed. Mater. Res., 32, 401 (1996) 
  39. Iwasaki Y, Ishihara K, Nakabayashi N, Khang G, Jeon JH, Lee JW, Lee HB, J. Biomater. Sci.-Polym. Ed., 9, 801 (1998)
  40. Iwasaki Y, Sawada S, Nakabayashi N, Khang G, Lee HB, Ishihara K, Biomaterials, 20, 2185 (1999) 
  41. Khang G, Lee JW, Jeon JH, Lee JH, Lee HB, Biomaterials Res., 1, 1 (1997)
  42. Lee JH, Khang G, Lee JW, Lee HB, J. Colloid Interface Sci., 205(2), 323 (1998) 
  43. Grabowski EF, Herther KK, Didisheim P, J. Lab. Clin. Med., 88, 368 (1976)
  44. Park K, Mao FW, Park H, Biomaterials, 11, 24 (1990) 
  45. Park K, Park H, Scanning Microscopy, 3, 137 (1989)
  46. van Kooten TG, Schakenraad JM, van der Mei HC, Busscher HJ, J. Biomed. Mater. Res., 26, 725 (1992) 
  47. Hasegawa T, Iwasaki Y, Ishihara K, Biomaterials, 22, 243 (2001) 
  48. Lin JC, Ko TM, Cooper SL, J. Colloid Interface Sci., 164(1), 99 (1994) 
  49. Ko TM, Lin JC, Cooper SL, J. Colloid Interface Sci., 156, 207 (1993) 
  50. Neumann AW, Absolom DR, Zingg W, van Oss CJ, Francis DW, Surface Thermodynamics of Protein Adsorption, Cell Adhesion and Cell Engulfment, in Biocompatible Polymers, Metals, and Composites, M. Szycher, Ed., Technomic, Lancaster, Pennsylvania, pp 275-299. (1986)
  51. Andrade JD, Principles of Protein Adsorption, in Surface and Interfacial Aspects of Biomedical Polymers, J.D. Andrade, Ed., Plenum, NY-London, Vol. 2, pp 1-80. (1985)
  52. Andrade JD, Hlady V, Adv. Polym. Sci., 79, 1 (1986)