Korean Journal of Chemical Engineering, Vol.18, No.3, 357-362, May, 2001
Comparison of the Adsorption Characteristics of Expanded Bed Adsorbent with Conventional Chromatographic Adsorbent
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The static and kinetic adsorption characteristics of Streamline DEAE and DEAE-Sepharose FF were studied under various operating conditions. The adsorption isotherms for the two types of adsorbents were obtained and found to fit well to a Langmuir-type expression. The adsorption kinetics of Streamline DEAE at different concentrations, temperatures, and viscosities were studied and a mathematical model including particle size distribution was developed to describe the adsorption performance of Streamline DEAE. Comparing the uptake curves of Streamline DEAE with DEAE-Sepharose FF, it could be concluded that Streamline DEAE achieves equilibrium faster to get equilibrium than DEAE-Sepharose FF, indicating that Streamline DEAE could be used in higher flow rate systems.
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Chang YK, Chase HA, Biotechnol. Bioeng., 49(5), 512 (1996)
- Chase HA, J. Chromatogr., 297, 179 (1984)
- Chase HA, Dreager MN, J. Chromatogr., 597, 129 (1992)
- Chase HA, Trends Biotechnol., 12, 296 (1994)
- Cho D, Cornec M, Korean J. Chem. Eng., 16(3), 371 (1999)
- Choi JW, Korean J. Chem. Eng., 7(4), 269 (1990)
-
Clemmitt RH, Chase HA, Biotechnol. Bioeng., 67(2), 206 (2000)
- Finlayson BA, "Nonlinear Analysis in Chemical Engineering," McGraw-Hill Inc., New York (1980)
- Graham EE, Fook CH, AIChE J., 28, 245 (1982)
- Hjorth R, Trends Biotechnol., 15, 230 (1997)
- Horstmann BJ, Chase HA, Chem. Eng. Res. Des., 67, 243 (1989)
- Hu HB, Yao SJ, Zhu ZQ, Chem. Eng.-Chinese, 27, 37 (1999)
- Johnston A, Hearn MTW, J. Chromatogr., 512, 101 (1990)
- Johnston A, Mao QM, Hearn MTW, J. Chromatogr., 548, 127 (1991)
-
Karau A, Benken C, Thommes J, Kula MR, Biotechnol. Bioeng., 55(1), 54 (1997)
-
Mullick A, Flickinger MC, Biotechnol. Bioeng., 65(3), 282 (1999)
-
Owen RO, Chase HA, Chem. Eng. Sci., 54(17), 3765 (1999)
- Park IS, Kwak C, Hwang YG, Korean J. Chem. Eng., 17(6), 704 (2000)
- Skidmore GL, Horstmann BJ, Chase HA, J. Chromatogr., 498, 113 (1990)
- Tsou HS, Graham EE, AIChE J., 31, 1959 (1985)
-
Wright PR, Muzzio FJ, Glasser BJ, Biotechnol. Prog., 15(5), 932 (1999)