화학공학소재연구정보센터
Biotechnology and Bioengineering, Vol.67, No.6, 726-736, 2000
The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels (Reprinted from Biotechnology and Bioengineering, vol 19, pg 387-397, 1977)
Saccharomyces cerevisiae cells, Kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels. Ethanol production from glucose solutions by an immobilized preparation of S. cerevisiae was demonstrated over a total of twenty-three days, and the half-life of such a preparation was shown to be about ten days. Immobilized K. marxianus, inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining properties of calcium alginate gels. Calcium alginate-immobilized chloroplasts were shown to perform the Hill reaction. Some experiments with immobilized mitochondria are reported.