Biotechnology Letters, Vol.16, No.3, 229-234, 1994
Investigation of Apigenin-7-0-Beta-Glucoside Hydrolysis by Beta-Glucosidase from Almonds
The enzymatic transformation of apigenin-7-O-beta-glucoside into apigenin is effected using beta-glucosidase from almonds. Kinetic studies gave the following values K-m=7.63x10(-4)mol/dm(3) -1 -1 m v(max)=10.37 mu mol mg(-1) min(-1) and E(1)=65.81 kJ/mol when the substrate was pure apigenin-7-O-beta-glucoside, while in case of apigenin-7-O-beta glucoside from dry extract of camomile ligulate flowers E(2)=65.57 kJ/mol.