Biotechnology Letters, Vol.16, No.12, 1275-1280, 1994
Elicitation of Anthocyanin Production in Cell-Cultures of Carrot (Daucus-Carota L) by Using Elicitors and Abiotic Stress
A cell line of carrot (Daucus carota L) which produces anthocyanin was subjected to various elicitors and abiotic stresses. The elicitors tested were culture filtrates (CF) and cell extracts (CE) of certain bacteria and yeasts. The abiotic stresses were salts of certain metal ions. The production increase obtained with cell extracts of Bacillus cereus. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were 49, 72, 45 and 41% respectively over the control. Maximum elicitation was obtained with elicitor derived from cell extract of the yeast Rhodotorula rubra where it enhanced production by two fold. The abiotic stress agents Ca, Mn, Zn, Co, Fe & V enhanced anthocyanin production. Of all the metal ions tested Ca was the most effective. The elicitation process was governed by the type and level of elicitor.