An electrochemical model for a microbial fuel cell process is proposed here. The model was set up on the basis of the experimental results and analysis of biochemical and electrochemical processes. Simulation of the process shows that the model describes the process reasonably well. The analysis of model simulation illustrates how the current output depends on the substrate concentration, mediator concentration and other main variables. The relationship between the current output and over-voltage is revealed from the modelling study.