The role of laccase in ligninolysis has been disputed but it still seems to have an important place in the reactions. Trametes versicolor has been the organism of choice for laccase production. The present paper reports laccase production by Phlebia fascicularia, P. floridensis and Dichomitus squalens. Enzyme production in mineral salts broth, malt extract broth and in the presence of various supplements showed that P. floridensis and D. squalens were better laccase producers than T. versicolor. Different lignin preparations and natural agricultural residues gave the best enzyme production, except with T. versicolor. The studies underline the need to explore more organisms to fully exploit the potential of laccase-producing fungi.