The nutrient portion of the Groundwater Loading Effects of Agricultural Management practices (GLEAMS) model was analyzed under Mid-Atlantic conditions at a municipal wastewater land treatment facility. A sensitivity analysis was performed on soil nitrate concentration and crop nitrogen uptake over model input variables. Input variables having a relatively significant effect on the two outputs of interest were identified for adjustment during model calibration. Calibration was performed in an attempt to adjust model output as closely as possible to measured field data. GLEAMS was not satisfactorily calibrated for Mid-Atlantic conditions. Failure of the model to properly mineralize organic nitrogen was identified.