A novel method of citric acid production from beet molasses was developed, in which an anion-exchange resin packed-column was connected to a fermenter for separation of citric acid from fermentation broth. The experimental results indicated that, as compared with conventional batch, the new fermentation techniques increased the citric acid productivity and sugar conversion from 0.338 g/l h and 82.2% to 0.543 g/l h and 94.8%, respectively.