화학공학소재연구정보센터
Biotechnology Letters, Vol.22, No.8, 647-650, 2000
Overproduction of an extracellular polysaccharide possessing high lipid emulsion stabilizing effects by Bacillus sp.
Bacillus sp. CP912, producing an extracellular biopolymer, was isolated from the soil. Maximum accumulation of the biopolymer was 10 g l(-1) culture broth with a yield of 88% from glucose consumed. The biopolymer was purified with several precipitation steps using ethanol and cetyl-trimethyl-ammonium bromide. Carbohydrate analyses using various color reactions, infrared spectroscopy, and high performance liquid chromatography revealed that the biopolymer is a homopolysaccharide. The lipid emulsifying capacity of the polysaccharide was 100%, while that of xanthan gum was 94%.