The new fructosyltransferase (FTase) from Bacillus macerans EG-6 showed a broad acceptor specificity, and resulted in the formation of fructosylxyloside (FX) with D-xylose being the most effective acceptor. The optimal FTase concentration for FX production was 0.6 unit per g sucrose, which gave the highest transfer ratio, 83%, of fructosyl moiety from sucrose to D-xylose. Maximum yield of FX was 114 g l(-1) with 200 g sucrose l(-1) and 300 g D-xylose l(-1).