Biotechnology Letters, Vol.23, No.1, 17-20, 2001
Evaluation of transcript level and biomass during mycelial development of the truffle, Tuber borchii
The growth of the truffle, Tuber borchii, mycelial strain (1BO) was analysed using biomolecular methods. Total RNA evaluation and ergosterol assay revealed that transcriptional and metabolic activity reach a peak at around 30-50 days of growth and decrease by 70 days. mRNA electrophoretic profiles highlighted a very low degree of variation in gene expression during the growth of mycelium.